The chemistry is validated down to a 500 pg floor and runs in a single tube from input to sequencing-ready in two hours. We ship evaluation kits free so you can prove it against the chemistry you run now, with no purchase, no quote, and no sales call.
Every kit has a number on the datasheet. The question is what reaches the flow cell.
No purchase. No quote. No sales call.Kits shipped free, plus the technical note instantly.
1.75% → 0.87%
After a 62-sample pilot, Trisomy test Ltd verified the switch on 2,772 production samples and watched their uninformative rate fall by half - answers on samples that should have worked all along. Library construction dropped from 5 hours 20 to 4 hours 20, which puts plasma to sequencer inside a working day, and at their volume that works out to 93 more answered cases every year.
Source: Watchmaker NIPT case study.
15/16
Nine paediatric tumour biopsies went through four stranded RNA workflows in the hands of four different technicians. The Watchmaker libraries caught 15 of 16 Seraseq control fusions at both standard and low input, where the comparators caught 14 and 9, and they did it with the least hands-on time of the four. Those libraries then ran the hospital’s existing EnFusion pipeline unmodified and clustered by biology rather than by chemistry against a transcriptome database built up over years on the previous kit.
Source: Dr. Katie Miller, AMP 2024.
20+
Sanger benchmarked more than twenty high-fidelity enzymes for NGS library amplification in a peer-reviewed study and named three. Equinox was one of them, and we did not run, fund or review the work.
Source: Quail et al., Microbial Genomics, April 2024.
Trisomy test Ltd piloted the chemistry on 62 samples, then verified it on 2,772 more before moving their NIPT assay across - and the uninformative rate halved. That is the standard of proof this kit has already cleared.
The floor is validated at 500 pg, the yield is 9x at 1 ng without adding a single PCR cycle, and chimeric and hairpin artifacts drop by up to 90% - so a structural variant call becomes a finding instead of a confirmation queue item.
You run it on your own samples, your own deck and your own pipeline, and a scientist reviews your input range before anything ships. If a different chemistry fits you better, we say so.
A cfDNA draw at 11 weeks, an FFPE block that’s the last tissue there is, a biopsy that cost a scheduled procedure - the protocol wants 50 to 200 ng and the tube holds a fraction of it. You run it anyway.
You get 9x at 1 ng, and Equinox Prime showed the lowest error of the amp mixes benchmarked - up to 55% below the highest-error mix.
This is the only prep tested low in both AT-rich and GC-rich dropout, and it held that at 75 ng while every comparator ran at 300 ng.
Two hours in a single tube, with automation methods published for SciClone G3 NGSx, Biomek i7 and Hamilton Polaris.
Dropout is genome you paid to sequence and did not receive.
The same chemistry covers cfDNA and liquid biopsy, FFPE and degraded RNA, PCR-free WGS, methylation without bisulfite conversion, and targeted capture, ChIP, metagenomics and viral work - with input floors from 500 pg on the DNA side and 1 ng on RNA with Polaris depletion. One dropdown on the form covers all of it. A scientist reads your input range against the chemistry before anything ships, and if ours isn’t the right fit, we tell you before you spend a run finding out.
No purchase, no quote, no sales call.
1. Start with two fields - your sample type and typical input range. 2. A scientist reviews your protocol and ships kits matched to your samples, free, scientist to scientist. 3. Run the side-by-side on your own deck with your own pipeline and metrics. It’s one day of bench work, and you decide when your data says so.
Every kit ships with the four-page technical note (which downloads the moment you submit), the Trisomy ESHG 2024 recording, SciLifeLab’s PCR-free method for the NovaSeq X, the technical webinar behind the artifact and error data, and rare-variant and FFPE data we haven’t published on this page - full methods included.
No purchase, no quote, no sales call.
Our founders co-founded two of the businesses whose enzymes this field standardised on, and led R&D at a third. They built the products the field runs on - then rebuilt the enzymes at the point where those products lose data, which is why the margin shows up on cfDNA, FFPE and low input rather than on a clean gDNA control. The enzymes are engineered by directed evolution and manufactured in-house under ISO 13485:2016.
Every lot is released on functional testing rather than analytical spec alone, because that’s what actually holds lot-to-lot behaviour steady inside an assay - and every shipment comes from a single lot, so you QC once regardless of order size. Manufacturing runs under a BSI-certified ISO 13485:2016 quality system, certificate FM 762818, current to January 2029, and you can validate the number yourself at bsigroup.com/ClientDirectory. Custom and OEM work is a core business rather than a favour: lyophilization, tailored fill volumes, private and white label, and bulk fills, running four to eight weeks from PO to delivery with a dedicated project manager and minimums sized for smaller groups.
Stocked at the Harvard Biopolymers Facility and the MIT Genome Technology Core. Nine commercial partners build their own products on these enzymes.
Technology and supply partners
| Claim | Result | Method | Whose data |
|---|---|---|---|
| Library yield | 9x at 1 ng | Triplicate, 1 ng Isopure cfDNA, each kit on its own protocol and adapter concentration | Ours |
| Dedup UMI coverage | +20-30% | HD779 Horizon, 0.1% AF, 12-plex 37 kb oncology panel, NovaSeq, 25M read pairs | Ours |
| Polymerase error | Up to 55% lower | Equinox Prime vs three commercial amp mixes, per substitution type; 55% vs the highest-error mix, narrower vs the closest | Ours |
| Chimeric + hairpin artifacts | Up to 90% fewer | Duplicate libraries at 1, 10, 50, 100 ng gDNA vs two enzymatic frag kits plus sonication | Ours |
| Control fusions detected | 15/16 | Seraseq Fusion RNA Mix v4, standard and low input | Nationwide Children’s |
| Production verification | n=2,772 | Production NIPT following a 62-sample pilot | Trisomy test Ltd |
| Enzyme benchmark | One of three named of 20+ | Peer-reviewed; we did not run, fund or review it | Sanger (Quail et al., 2024) |
No, and it’s been tested at scale. Nationwide Children’s ran Watchmaker libraries through their existing EnFusion pipeline without modifying it, then compared gene expression against a transcriptome database built up over years on their previous chemistry. The samples clustered by biological profile rather than by library chemistry - which is exactly what no batch effect looks like.
The DNA kit is validated down to 500 pg, and Trisomy test Ltd ran cfDNA at inputs down to 0.5 ng across their entire 2,772-sample production verification. The DNA Library Prep Kit is specified from 500 pg to 1 µg, the version with Fragmentation runs from under 1 ng to 500 ng, and on the RNA side Polaris Depletion runs from 1 ng with total RNA from 0.25 ng.
Nothing. You get enough reagent for a real side-by-side against your current kit on your own samples, a protocol review with one of our scientists, and the full benchmarking package with methods. Contents depend on your sample type and input range, which is why the form asks. Catalogue pricing is quoted after evaluation because it varies too widely by product and volume for a single number here to be honest - though Trisomy test Ltd cited competitive pricing as one of their three reasons for moving.
Manufacturing sits inside a BSI-certified ISO 13485:2016 quality system, certificate FM 762818, and every lot is released against functional testing rather than analytical specification alone. Shipments come from a single lot. In the Nationwide Children's Hospital study, 18 libraries prepared by four different technicians showed no correlation between operator and outcome, and Trisomy measured a significantly narrower final library concentration distribution than their reference workflow, which removed the need to re-dilute concentrated libraries before pooling.
Yes, and it is a core business rather than a favour. Custom formulations including lyophilization, tailored fill volumes, private and white label, branded ready-to-sell kits, and bulk fills for customer-managed fill and finish. Purchase order to delivery runs 4 to 8 weeks for complex customized private-label kits, with a dedicated project manager, flexible terms and accommodating minimum volumes for smaller organizations.
Kits ship worldwide from Boulder, Colorado, either direct or through the distributor network, so an EMEA address is not a problem. Contents are matched to the sample type and throughput you give us on the form, and a scientist reviews your protocol before anything is packed.
Yes. Watchmaker products are labelled For Research Use Only and are not for use in diagnostic procedures. The quality system is ISO 13485:2016 certified, which supports customers developing their own regulated assays, but that certification applies to our manufacturing rather than converting our products into cleared diagnostics.
Your uninformative rate isn’t a constant - it’s the output of a chemistry decision most labs made once and never revisited, because revalidation looked more expensive than the loss.
Trisomy test Ltd revisited it and gained 93 answered cases a year. Nationwide Children’s revisited it and got a day back on every batch without touching their pipeline.
Both of them started exactly where this form starts: free kits and their own samples.
No purchase, no quote, no sales call.Kits ship free, and the technical note downloads instantly.